Affiliation:
1. Department of Endodontics, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland
2. Division of Biophysics, Institute of Materials Science and Engineering, Lodz University of Technology, ul Stefanowskiego 1/15, 90-924 Lodz, Poland
Abstract
The aim of this study was to determine the bactericidal potential of graphene oxide (GO) in contact with four species of bacteria: E. coli, S. mutans, S. aureus and E. faecalis. Bacterial cell suspensions of each species were incubated in a medium containing GO, with incubation times of 5, 10, 30 and 60 min, at final concentrations of 50, 100, 200, 300 and 500 μg/mL. The cytotoxicity of GO was evaluated using live/dead staining. The results were recorded using a BD Accuri C6 flow cytofluorimeter. Obtained data were analyzed using BD CSampler software. A significant bacteria viability reduction was noted in all GO-containing samples. The antibacterial properties of GO were strongly influenced by GO concentration and incubation time. The highest bactericidal activity was observed at concentrations of 300 and 500 μg/mL for all incubation times (5, 10, 30 and 60 min). The highest antimicrobial potential was observed for E. coli: after 60 min, the mortality rate was 94% at 300 µg/mL GO and 96% at 500 µg/mL GO; the lowest was found for S. aureus—49% (300 µg/mL) and 55% (500 µg/mL).
Funder
Medical University of Lodz
Subject
General Materials Science
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