Author:
Zhou Ting,Liu Mengting,Pan Jialiang,Ren Jianluan,Tang Fang,Dai Jianjun,Xue Feng,Ji Dejun
Abstract
Background: With the discovery of more and more drug–resistant bacterial strains, there is an urgent need for safer and more effective alternative treatments. In this study, antibacterial peptides and probiotic microcapsules were combined to treat gastrointestinal inflammation caused by Vibrio parahaemolyticus infection. Methods: To improve the stability of probiotics in the gastrointestinal tract, two types of mixed natural anionic polysaccharides and chitosan were used as carriers to embed the probiotics. Taking Lacticaseibacillus casei CGMCC1.8727 microcapsules with good performance as the research object, the in vitro characteristics of the microcapsules were studied via acid resistance test and intestinal release test. The microcapsules were then tested for in vivo treatment in combination with the antibacterial peptide, bomidin, and the therapeutic effects were compared among microencapsulated probiotics, free probiotics, and probiotics in combination with bomidin. Results: Microencapsulation was successfully manufactured under suitable processing parameters, with the product particle size being 2.04 ± 0.2743 mm. Compared with free probiotics, microencapsulation significantly improved the activity and preservation stability of the probiotics under simulated gastrointestinal conditions. Microencapsulated probiotics showed better therapeutic effects than free probiotics in vivo. Microcapsules combined with antimicrobial peptides accelerated the elimination of bacteria in vivo. This study provides a reference for anti–inflammatory treatment, especially for the treatment of gastrointestinal diseases.
Funder
Ministry of Science and Technology of China: National Key R&D Program Project
Subject
Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics
Reference36 articles.
1. Vibrio parahaemolyticus: A review on the pathogenesis, prevalence, and advance molecular identification techniques;Letchumanan;Front. Microbiol.,2014
2. Design and application of conjugated polymer nanomaterials for detection and inactivation of pathogenic microbes;Li;ACS Appl. Bio Mater.,2020
3. Krawczyk, B., Wityk, P., Gałęcka, M., and Michalik, M. The many faces of Enterococcus spp.—Commensal, probiotic and opportunistic pathogen. Microorganisms, 2021. 9.
4. A taxonomic note on the genus lactobacillus: Description of 23 novel gene–ra, emended description of the genus lactobacillus beijerinck 1901, and union of lactobacillaceae and leuconostoca–ceae;Zheng;Int. J. Syst. Evol. Microbiol.,2020
5. Zhao, Y.M. A probiotic Composition Having an Anti–Sensitization Effect. Sichuan Province. 2021.