Abstract
Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response.
Subject
Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics
Reference53 articles.
1. Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology;Front. Phys.,2021
2. FAO (2022, August 08). News Article: Pollutants from Agriculture a Serious Threat to World’s Water. Available online: https://www.fao.org/news/story/en/item/1141534/icode/.
3. (2022, August 08). Land Is a Critical Resource, IPCC Report Says—IPCC. Available online: https://www.ipcc.ch/2019/08/08/land-is-a-critical-resource_srccl/.
4. Effect of plasma treatment on the seed germination and seedling growth of radish (Raphanus sativus);Plasma Sci. Technol.,2022
5. Cold plasma seed treatment improves chilling resistance of tomato plants through hydrogen peroxide and abscisic acid signaling pathway;Free Radic. Biol. Med.,2021
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献