Technology Invention and Mechanism Analysis of Rapid Rooting of Taxus × media Rehder Branches Induced by Agrobacterium rhizogenes

Author:

Wang Ying12ORCID,Luo Xiumei1ORCID,Su Haotian13,Guan Ge13,Liu Shuang13,Ren Maozhi123

Affiliation:

1. Functional Plant Cultivation and Application Innovation Team, Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610230, China

2. Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China

3. School of Agricultural Science, Zhengzhou University, Zhengzhou 450052, China

Abstract

Taxus, a vital source of the anticancer drug paclitaxel, grapples with a pronounced supply–demand gap. Current efforts to alleviate the paclitaxel shortage involve expanding Taxus cultivation through cutting propagation. However, traditional cutting propagation of Taxus is difficult to root and time-consuming. Obtaining the roots with high paclitaxel content will cause tree death and resource destruction, which is not conducive to the development of the Taxus industry. To address this, establishing rapid and efficient stem rooting systems emerges as a key solution for Taxus propagation, facilitating direct and continuous root utilization. In this study, Agrobacterium rhizogenes were induced in the 1–3-year-old branches of Taxus × media Rehder, which has the highest paclitaxel content. The research delves into the rooting efficiency induced by different A. rhizogenes strains, with MSU440 and C58 exhibiting superior effects. Transcriptome and metabolome analyses revealed A. rhizogenes’ impact on hormone signal transduction, amino acid metabolism, zeatin synthesis, and secondary metabolite synthesis pathways in roots. LC-MS-targeted quantitative detection showed no significant difference in paclitaxel and baccatin III content between naturally formed and induced roots. These findings underpin the theoretical framework for T. media rapid propagation, contributing to the sustainable advancement of the Taxus industry.

Funder

Sichuan Science and Technology Program

Central Public-Interest Scientific Institution Basal Research Fund

Agricultural Science and Technology Innovation Program

Nanfan Research Institute

National key R&D program of China

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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