Protective Effect of Aquilaria crassna Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and Caenorhabditis elegans: Possible Active Constituent Includes Clionasterol
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Published:2023-09-14
Issue:18
Volume:15
Page:3985
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Pattarachotanant Nattaporn12ORCID, Rangsinth Panthakarn3ORCID, Warayanon Watis12ORCID, Leung George Pak-Heng3ORCID, Chuchawankul Siriporn4ORCID, Prasansuklab Anchalee15ORCID, Tencomnao Tewin12ORCID
Affiliation:
1. Natural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Chulalongkorn University, Bangkok 10330, Thailand 2. Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3. Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China 4. Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand 5. College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Abstract
Aquilaria crassna (AC) is a beneficial plant widely used to alleviate various health ailments. Nevertheless, the neuroprotection, antiaging, and xenobiotic detoxification against high benzo[a]pyrene induction have not been investigated. This study aimed to investigate the effects of ethanolic extract of AC leaves (ACEE) in vitro using SH-SY5Y cells and in vivo using Caenorhabditis elegans (C. elegans). Neuroprotective activities and cell cycle progression were studied using SH-SY5Y cells. Additionally, C. elegans was used to determine longevity, health span, and transcriptional analysis. Furthermore, ACEE possible active compounds were analyzed by gas chromatograph–mass spectrometry (GC-MS) analysis and the possible active compounds were evaluated using a molecular docking study. First, ACEE possessed neuroprotective effects by normalizing cell cycle progression via the regulation of AhR/CYP1A1/cyclin D1 pathway. Next, ACEE played a role in xenobiotic detoxification in high B[a]P-induced C. elegans by the amelioration of lifespan reduction, and body length and size decrease through the reduction in gene expression in hexokinase (hxk) and CYP35 pathway. Finally, phytochemicals of ACEE were identified and we uncovered that clionasterol was the possible active constituent in powerfully inhibiting both CYP1A1 and hexokinase II receptor. Essentially, ACEE was recognized as a potential alternative medicine to defend against high B[a]P effects on neurotoxicity and xenobiotic detoxification.
Funder
Thailand Science research and Innovation Fund, Chulalongkorn University
Subject
Food Science,Nutrition and Dietetics
Reference80 articles.
1. De Rubis, G., Paudel, K.R., Manandhar, B., Singh, S.K., Gupta, G., Malik, R., Shen, J., Chami, A., MacLoughlin, R., and Chellappan, D.K. (2023). Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1. 1 Airway Epithelial Cells. Nutrients, 15. 2. Assessment of the bioactive components, antioxidant, antiglycation and anti-inflammatory properties of Aquilaria crassna Pierre ex Lecomte leaves;Wongwad;Ind. Crops Prod.,2019 3. Pattarachotanant, N., Sornkaew, N., Warayanon, W., Rangsinth, P., Sillapachaiyaporn, C., Vongthip, W., Chuchawankul, S., Prasansuklab, A., and Tencomnao, T. (2022). Aquilaria crassna leaf extract ameliorates glucose-induced neurotoxicity in vitro and improves lifespan in caenorhabditis elegans. Nutrients, 14. 4. Lamptey, R.N., Chaulagain, B., Trivedi, R., Gothwal, A., Layek, B., and Singh, J. (2022). A review of the common neurodegenerative disorders: Current therapeutic approaches and the potential role of nanotherapeutics. Int. J. Mol. Sci., 23. 5. Erythropoietin: A neuroprotective agent in cerebral hypoxia, neurodegeneration, and epilepsy;Merelli;Curr. Pharm. Des.,2013
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