Effects of Selenium in Different Valences on the Community Structure and Microbial Functions of Biofilms

Abstract

With the wide application of selenium (Se) in industrial production, different Se-based compounds (selenate and selenite) are produced and released into aquatic environments. The potential impacts of such Se compounds on the biofilms (a complex microbial aggregate in aquatic systems) need to be substantially explored. Herein, we investigated the responses of bacterial community diversity, composition and structure, and function of biofilms after 21 days of exposure to low concentrations (100 µg/L) and high concentrations (1 mg/L) of sodium selenate and sodium selenite, respectively. Distinct effects of selenium in different valences on the community structure and microbial functions of biofilms were observed. Compared with the controls, the addition of selenate and selenite solutions altered the richness of biofilms but not the diversity, which is dependent on the concentration and valences, with sodium selenite (1 mg/L) exhibiting a strong inhibition effect on community richness. Significant changes of community composition and structure were observed, with a significant increase in Proteobacteria (31.08–58.00%) and a significant decrease in Bacteroidetes (32.15–11.45%) after exposure to sodium selenite with high concentration. Also, different responses of gamma-Proteobacteria and alpha-Proteobacteria were observed between the sodium selenite and sodium selenate treatments. Moreover, results showed that sodium selenite could strengthen the function of the metabolism of biofilms, and the higher the concentration is, the more apparent the enhancement effect is. All these results suggested that the effects of different valence states of selenium were obvious, and sodium selenite with high concentration strongly changed the diversity, structure and function of biofilms.