Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources
Author:
Zhang Suilin12ORCID, Li Yang12, Li Yan12, Zhang Yunqi1ORCID, Hao Yanbin1, Hou Zhixia2, Qi Jianxun1
Affiliation:
1. Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China 2. State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China
Abstract
Walnut is one of four major nuts in the world. China has abundant walnut germplasm resources, but there are still shortcomings in the identification of germplasm resources. This study used different walnut varieties as materials and developed 14 high-quality SSR molecular markers from 60 pairs of primers based on genome re-sequencing results. This study analyzed the genetic diversity of Chinese walnut genetic resources using 14 selected SSR markers. A total of 64 alleles were detected in 47 walnut resources, with an average of 4.571 alleles per locus. The variation range of polymorphism information content was 0.096~0.711, with an average value of 0.422. Cluster analysis, population genetic structure, and principal coordinate analysis divided 47 walnut resources into ordinary walnuts, Juglans hopeiensis, and Liaoyi 1. In addition, core SSR markers (Jr45, Jr40, Jr29, Jr35, and Jr11) were selected from 14 SSR markers, which were sufficient to distinguish 47 walnut resources. At the same time, 47 unique molecular fingerprints of walnuts were constructed using these core SSR markers. This study provides strong scientific support for rapid and efficient identification, germplasm innovation, and a variety of property protection of walnut germplasm.
Funder
Innovation Capacity Construction Project of Beijing Academy of Agricultural and Forestry Sciences Youth Fund of Beijing Academy of Forestry and Pomology Sciences
Reference53 articles.
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