CsCuAO1 Associated with CsAMADH1 Confers Drought Tolerance by Modulating GABA Levels in Tea Plants

Author:

Cao Yu1,Chen Yiwen1,Cheng Nuo1,Zhang Kexin1,Duan Yu1,Fang Shimao12,Shen Qiang2,Yang Xiaowei2,Fang Wanping1,Zhu Xujun1ORCID

Affiliation:

1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

2. Tea Research Institute, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 417100, China

Abstract

Our previous study showed that COPPER-CONTAINING AMINE OXIDASE (CuAO) and AMINOALDEHYDE DEHYDROGENASE (AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea through the polyamine degradation pathway. However, their biological function in drought tolerance has not been determined. In this study, Camellia sinensis (Cs) CsCuAO1 associated with CsAMADH1 conferred drought tolerance, which modulated GABA levels in tea plants. The results showed that exogenous GABA spraying effectively alleviated the drought-induced physical damage. Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine by stimulating reactive oxygen species’ scavenging capacity and stomatal movement. However, the suppression of CsCuAO1 or CsAMADH1 in tea plants resulted in increased sensitivity to drought treatment. Moreover, co-overexpressing plants increased GABA accumulation both in an Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter gene, CsGAT1, was identified, whose expression was strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in the response to drought stress through a dynamic GABA-putrescine balance. Our data will contribute to the characterization of GABA’s biological functions in response to environmental stresses in plants.

Funder

Natural Science Foundation of Guizhou Province

National Natural Science Foundation of China

China Agriculture Research System of MOF and MARA

Changzhou Agriculture Science and Technology Support Program

Research Students Training Project of Nanjing Agricultural University

Guizhou Provincial Academy of Agricultural Sciences

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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