Bacteriophage T5 dUTPase: Combination of Common Enzymatic and Novel Functions
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Published:2024-01-10
Issue:2
Volume:25
Page:892
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Glukhov Anatoly1, Marchenkov Victor1ORCID, Dzhus Ulyana1, Krutilina Antonina1, Selikhanov Georgii123ORCID, Gabdulkhakov Azat1ORCID
Affiliation:
1. Institute of Protein Research RAS, 142290 Pushchino, Russia 2. International Institute “Solution Chemistry of Advanced Materials and Technologies”, ITMO University, 191002 St. Petersburg, Russia 3. Almetyevsk State Petroleum Institute, 423450 Almetyevsk, Russia
Abstract
The main function of dUTPases is to regulate the cellular levels of dUTP and dTTP, thereby playing a crucial role in DNA repair mechanisms. Despite the fact that mutant organisms with obliterated dUTPase enzymatic activity remain viable, it is not possible to completely knock out the dut gene due to the lethal consequences of such a mutation for the organism. As a result, it is considered that this class of enzymes performs an additional function that is essential for the organism’s survival. In this study, we provide evidence that the dUTPase of bacteriophage T5 fulfills a supplemental function, in addition to its canonical role. We determined the crystal structure of bacteriophage T5 dUTPase with a resolution of 2.0 Å, and we discovered a distinct short loop consisting of six amino acid residues, representing a unique structural feature specific to the T5-like phages dUTPases. The removal of this element did not affect the overall structure of the homotrimer, but it had significant effects on the development of the phage. Furthermore, it was shown that the enzymatic function and the novel function of the bacteriophage T5 dUTPase are unrelated and independent from each other.
Funder
Russian Science Foundation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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