Affiliation:
1. Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, 10223 Vilnius, Lithuania
Abstract
Azo dyes are of concern due to their harmful effects on the environment and human health. The oxidation of 2,2′-dihydroxyazobenzene (DHAB) catalyzed with recombinant Coprinus cinereus (rCiP) peroxidase was investigated. The kinetic measurements were performed using the spectrophotometric and fluorimetric methods. The dependences of the initial reaction rates on enzyme, substrate and hydrogen peroxide concentrations during DHAB oxidation were established, and bimolecular constants of enzyme interaction with DHAB were calculated. This research demonstrated that the initial biocatalytic oxidation rates of DHAB depend on the pH and the estimated pKa values of the active forms of rCip. This study’s findings thus contribute to a more comprehensive understanding of the biocatalytic oxidation of DHAB, providing valuable data for assessing the long-term toxicity, carcinogenesis and epigenetic effects of azo dyes in the environment.
Reference33 articles.
1. Ataei, M., Maghsoudi, A.S., and Hassani, S. (2022). Reference Module in Biomedical Sciences, Elsevier.
2. Classifications, properties and applications of textile dyes: A review;Benkhaya;Appl. J. Environ. Eng. Sci.,2017
3. Wexler, P. (2014). Encyclopedia of Toxicology, Academic Press. [3rd ed.].
4. Recent Applications of Azo Dyes: A Paradigm Shift from Medicinal Chemistry to Biomedical Sciences;Khan;Mini Rev. Med. Chem.,2021
5. Classifications, properties, recent synthesis and applications of azo dyes;Benkhaya;Heliyon,2020