Whole-Cell Biosensor for Iron Monitoring as a Potential Tool for Safeguarding Biodiversity in Polar Marine Environments
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Published:2024-06-28
Issue:7
Volume:22
Page:299
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ISSN:1660-3397
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Container-title:Marine Drugs
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language:en
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Short-container-title:Marine Drugs
Author:
Calvanese Marzia12ORCID, D’Angelo Caterina1ORCID, Tutino Maria Luisa12ORCID, Lauro Concetta1ORCID
Affiliation:
1. Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy 2. Istituto Nazionale Biostrutture e Biosistemi (I.N.B.B), Viale Medaglie D’Oro 305, 00136 Roma, Italy
Abstract
Iron is a key micronutrient essential for various essential biological processes. As a consequence, alteration in iron concentration in seawater can deeply influence marine biodiversity. In polar marine environments, where environmental conditions are characterized by low temperatures, the role of iron becomes particularly significant. While iron limitation can negatively influence primary production and nutrient cycling, excessive iron concentrations can lead to harmful algal blooms and oxygen depletion. Furthermore, the growth of certain phytoplankton species can be increased in high-iron-content environments, resulting in altered balance in the marine food web and reduced biodiversity. Although many chemical/physical methods are established for inorganic iron quantification, the determination of the bio-available iron in seawater samples is more suitably carried out using marine microorganisms as biosensors. Despite existing challenges, whole-cell biosensors offer other advantages, such as real-time detection, cost-effectiveness, and ease of manipulation, making them promising tools for monitoring environmental iron levels in polar marine ecosystems. In this review, we discuss fundamental biosensor designs and assemblies, arranging host features, transcription factors, reporter proteins, and detection methods. The progress in the genetic manipulation of iron-responsive regulatory and reporter modules is also addressed to the optimization of the biosensor performance, focusing on the improvement of sensitivity and specificity.
Reference229 articles.
1. Cochrane, S.K.J., Andersen, J.H., Berg, T., Blanchet, H., Borja, A., Carstensen, J., Elliott, M., Hummel, H., Niquil, N., and Renaud, P.E. (2016). What Is Marine Biodiversity? Towards Common Concepts and Their Implications for Assessing Biodiversity Status. Front. Mar. Sci., 3. 2. Heiskanen, A.-S., Berg, T., Uusitalo, L., Teixeira, H., Bruhn, A., Krause-Jensen, D., Lynam, C.P., Rossberg, A.G., Korpinen, S., and Uyarra, M.C. (2016). Biodiversity in Marine Ecosystems—European Developments toward Robust Assessments. Front. Mar. Sci., 3. 3. Composition of the Continental Crust;Rudnick;Treatise Geochem.,2003 4. What Controls Dissolved Iron Concentrations in the World Ocean? Authors’ Closing Comments;Johnson;Mar. Chem.,1997 5. The Integral Role of Iron in Ocean Biogeochemistry;Tagliabue;Nature,2017
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