Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture

Author:

Almeida Gustavo Henrique Doná Rodrigues1ORCID,da Silva-Júnior Leandro Norberto1ORCID,Gibin Mariana Sversut2ORCID,dos Santos Henrique2ORCID,de Oliveira Horvath-Pereira Bianca1,Pinho Leticia Beatriz Mazo1,Baesso Mauro Luciano2ORCID,Sato Francielle2ORCID,Hernandes Luzmarina3ORCID,Long Charles R.4,Relly Luciana4,Miglino Maria Angelica1,Carreira Ana Claudia Oliveira15

Affiliation:

1. Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil

2. Department of Physics, State University of Maringá, Maringá 87020-900, Brazil

3. Department of Morphological Sciences, State University of Maringa, Maringá 87020-900, Brazil

4. Department of Veterinary Physiology and Pharmacology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA

5. Centre for Natural and Human Sciences, Federal University of ABC, Santo André, São Paulo 09210-580, Brazil

Abstract

The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian tissue reconstruction was proposed to reestablish ovarian endocrine functions. Despite many decellularization methods proposed, there is no established protocol for whole ovaries by detergent perfusion that is able to preserve tissue macro and microstructure with higher efficiency. This generated biomaterial may have the potential to be applied for other purposes beyond reproduction and be translated to other areas in the tissue engineering field. Therefore, this study aimed to establish and standardize a protocol for porcine ovaries’ decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. For that, porcine ovaries (n = 5) were perfused with detergents (0.5% SDS and 1% Triton X-100) and submitted to an ultrasonication bath to produce acellular scaffolds. The decellularization efficiency was evaluated by DAPI staining and total genomic DNA quantification. ECM morphological evaluation was performed by histological, immunohistochemistry, and ultrastructural analyses. ECM physico-chemical composition was evaluated using FTIR and Raman spectroscopy. A cytocompatibility and cell adhesion assay using murine fibroblasts was performed. Results showed that the proposed method was able to remove cellular components efficiently. There was no significant ECM component loss in relation to native tissue, and the scaffolds were cytocompatible and allowed cell attachment. In conclusion, the proposed decellularization protocol produced whole-ovaries scaffolds with preserved ECM composition and great potential for application in tissue engineering.

Funder

The São Paulo Research Foundation

Coordination for the Improvement of Higher Education Personnel

CAPES PRINT

Publisher

MDPI AG

Subject

General Medicine

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