Human Oral Mucosa as a Potentially Effective Source of Neural Crest Stem Cells for Clinical Practice-Reference-Cited by-同舟云学术

Human Oral Mucosa as a Potentially Effective Source of Neural Crest Stem Cells for Clinical Practice

Published:2023-09-06 Issue:18 Volume:12 Page:2216
ISSN:2073-4409
Container-title:Cells
language:en
Short-container-title:Cells

Author:

Bardag Gorce Fawzia¹²³,Al Dahan Mais¹²,Narwani Kavita¹,Terrazas Jesus³,Ferrini Monica³,Calhoun Colonya C.¹⁴⁵⁶,Uyanne Jettie¹²⁷,Royce-Flores Jun¹²⁵,Crum Eric²⁴⁵,Niihara Yutaka¹⁸

Affiliation:

1. The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA

2. Division of Oral & Maxillofacial Surgery and Hospital Dentistry, Department of Surgery Harbor UCLA Medical Center, Torrance, CA 90502, USA

3. Charles R. Drew University of Medicine and Science, Los Angeles, CA 90059, USA

4. Department of Surgery, UCLA, David Geffen School of Medicine, Los Angeles, CA 90095, USA

5. UCLA School of Dentistry, Los Angeles, CA 90095, USA

6. Department of Oral & Maxillofacial Surgery and Hospital Dentistry, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA

7. Herman Ostrow School of Dentistry of USC, Los Angeles, CA 90089, USA

8. Emmaus Medical, Inc., Torrance, CA 90503, USA

Abstract

We report in this study on the isolation and expansion of neural crest stem cells (NCSCs) from the epithelium of oral mucosa (OM) using reagents that are GMP-certified and FDA-approved for clinical use. Characterization analysis showed that the levels of keratins K2, K6C, K4, K13, K31, and K15—specific to OM epithelial cells—were significantly lower in the experimental NCSCs. While SOX10 was decreased with no statistically significant difference, the earliest neural crest specifier genes SNAI1/2, Ap2a, Ap2c, SOX9, SOX30, Pax3, and Twist1 showed a trend in increased expression in NCSCs. In addition, proteins of Oct4, Nestin and Noth1 were found to be greatly expressed, confirming NCSC multipotency. In conclusion, our study showed that the epithelium of OM contains NCSCs that can be isolated and expanded with clinical-grade reagents to supply the demand for multipotent cells required for clinical applications in regenerative medicine. Supported by Emmaus Medical Inc.

Funder

Emmaus Medical Inc.

Publisher

MDPI AG

Subject

General Medicine

Link

https://www.mdpi.com/2073-4409/12/18/2216/pdf

Reference57 articles.

1. Salivary EGF regulates eosinophil-derived TGF-alpha expression in hamster oral wounds;Yang;Am. J. Physiol. Liver Physiol.,1996

2. Age and the architecture of oral mucosa;Sawair;Age,2012

3. López, S., Hoz, L., Tenorio, E.P., Buentello, B., Magaña, F.S., Wintergerst, A., Navas, A., Garfias, Y., and Arzate, H. (2021). Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?. Int. J. Mol. Sci., 22.

4. Use of a pre-vascularised oral mucosa cell sheet for promoting cutaneous burn wound healing;Lee;Theranostics,2018

5. Yamaguchi, N., Isomoto, H., Kobayashi, S., Kanai, N., Kanetaka, K., Sakai, Y., Kasai, Y., Takagi, R., Ohki, T., and Fukuda, H. (2017). Oral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosa dissection of squamous cell carcinoma and airplane transportation. Sci. Rep., 7.