Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc

Author:

Vlajić Tovilović Tamara1ORCID,Petrović Sanja1ORCID,Lazarević Miloš1ORCID,Pavić Aleksandar2,Plačkić Nikola2ORCID,Milovanović Aleksa3ORCID,Milošević Miloš3ORCID,Miletic Vesna4ORCID,Veljović Djordje5ORCID,Radunović Milena1ORCID

Affiliation:

1. School of Dental Medicine, University of Belgrade, 11 000 Belgrade, Serbia

2. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11 000 Belgrade, Serbia

3. Faculty of Mechanical Engineering, University of Belgrade, 11 000 Belgrade, Serbia

4. Faculty of Medicine and Health, Sydney Dental School, University of Sydney, Surry Hills, NSW 2010, Australia

5. Faculty of Technology and Metallurgy, University of Belgrade, 11 000 Belgrade, Serbia

Abstract

This study aimed to compare the biological properties of newly synthesized cements based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA). Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement (CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid (ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA, and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect (quantified by CFUs/mL) were assessed against Streptococcus mutans and Lactobacillus rhamnosus. None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation. CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against S. mutans and L. rhamnosus to MTA.

Funder

Ministry of Science, Technological Development and Innovation, Republic of Serbia

Publisher

MDPI AG

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