Genome-Wide Identification of Seven in Absentia E3 Ubiquitin Ligase Gene Family and Expression Profiles in Response to Different Hormones in Uncaria rhynchophylla
-
Published:2024-07-11
Issue:14
Volume:25
Page:7636
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Lan Jinxu1, Lian Conglong1ORCID, Shao Yingying2, Chen Suiqing1, Lu Ying2, Zhu Lina2, Mu Detian2, Tang Qi2ORCID
Affiliation:
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China 2. College of Horticulture, Hunan Agricultural University, Changsha 410128, China
Abstract
SINA (Seven in absentia) E3 ubiquitin ligases are a family of RING (really interesting new gene) E3 ubiquitin ligases, and they play a crucial role in regulating plant growth and development, hormone response, and abiotic and biotic stress. However, there is little research on the SINA gene family in U. rhynchophylla. In this study, a total of 10 UrSINA genes were identified from the U. rhynchophylla genome. The results of multiple sequence alignments and chromosomal locations show that 10 UrSINA genes were unevenly located on 22 chromosomes, and each UrSINA protein contained a SINA domain at the N-terminal and RING domains at the C-terminal. Synteny analysis showed that there are no tandem duplication gene pairs and there are four segmental gene pairs in U. rhynchophylla, contributing to the expansion of the gene family. Furthermore, almost all UrSINA genes contained the same gene structure, with three exons and two introns, and there were many cis-acting elements relating to plant hormones, light responses, and biotic and abiotic stress. The results of qRT-PCR show that most UrSINA genes were expressed in stems, with the least expression in roots; meanwhile, most UrSINA genes and key enzyme genes were responsive to ABA and MeJA hormones with overlapping but different expression patterns. Co-expression analysis showed that UrSINA1 might participate in the TIA pathway under ABA treatment, and UrSINA5 and UrSINA6 might participate in the TIA pathway under MeJA treatment. The mining of UrSINA genes in the U. rhynchophylla provided novel information for understanding the SINA gene and its function in plant secondary metabolites, growth, and development.
Reference51 articles.
1. Hypertension in China: Burdens, guidelines and policy responses: A state-of-the-art review;Yin;J. Hum. Hypertens.,2022 2. Uncaria rhynchophylla and its Major Constituents on Central Nervous System: A Review on Their Pharmacological Actions;Yang;Curr. Vasc. Pharmacol.,2020 3. Shao, Y., Zhou, Y., Yang, L., Mu, D., Wilson, I.W., Zhang, Y., Zhu, L., Liu, X., Luo, L., and He, J. (2024). Genome-wide identification of GATA transcription factor family and the effect of different light quality on the accumulation of terpenoid indole alkaloids in Uncaria rhynchophylla. Plant Mol. Biol., 114. 4. Mu, D., Shao, Y., He, J., Zhu, L., Qiu, D., Wilson, I.W., Zhang, Y., Pan, L., Zhou, Y., and Lu, Y. (2023). Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns of WRKY1 Transcription Factor and Rhynchophylline Biosynthesis-Related Genes in Uncaria rhynchophylla. Int. J. Mol. Sci., 24. 5. INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance;Cheng;Plant Physiol.,2018
|
|