Fir (Abies alba Mill.) Honeydew Honey Inhibits Growth and Adhesion of Campylobacter jejuni In Vitro-Reference-Cited by-同舟云学术

Fir (Abies alba Mill.) Honeydew Honey Inhibits Growth and Adhesion of Campylobacter jejuni In Vitro

Published:2023-11-28 Issue:23 Volume:13 Page:12735
ISSN:2076-3417
Container-title:Applied Sciences
language:en
Short-container-title:Applied Sciences

Author:

Ramić Dina¹,Gobin Ivana²^ORCID,Vučković Darinka²^ORCID,Planinić Ana³^ORCID,Brčić Karačonji Irena⁴⁵^ORCID,Smole Možina Sonja¹^ORCID

Affiliation:

1. Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamni karjeva 101, 1000 Ljubljana, Slovenia

2. Department of Microbiology, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, 51000 Rijeka, Croatia

3. Faculty of Health Studies, University of Mostar, Zrinskog Frankopana 34, 88000 Mostar, Bosnia and Herzegovina

4. Division of Toxicology, Institute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, Croatia

5. Faculty of Health Studies, University of Rijeka, Viktora Cara Emina 5, 51000 Rijeka, Croatia

Abstract

This study investigated the physicochemical properties of fir (Abies alba Mill.) honeydew honey (FHH) and its biological activity against the leading foodborne bacterium Campylobacter jejuni. Antibacterial activity, bacterial growth and anti-adhesion tests were performed in Mueller Hinton (MH) broth and cow’s milk used as a food model. FHH from the mountainous region of Croatia showed antibacterial activity against C. jejuni in MH broth and milk, with the minimal inhibitory concentration (MIC) values ranging from 12.5 to 50 mg/mL. A transmission electron microscopy (TEM) analysis of C. jejuni cells showed severe cellular damage after exposure to the tested honeydew honey. FHH in concentrations of 3 and 5% (w/v) inhibited growth and prevented adhesion to the polystyrene surface at a concentration of 25 mg/mL or lower of the bacterium C. jejuni in MH broth and milk. The anti-adhesion effect was more pronounced in the milk than in the MH broth. These results suggest that FHH has potent antibacterial activity against C. jejuni due to its particular physicochemical properties.

Funder

University of Rijeka

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

Link

https://www.mdpi.com/2076-3417/13/23/12735/pdf

Reference57 articles.

1. Fernández-Estellé, M., Hernández-González, V., Saurina, J., Núñez, O., and Sentellas, S. (2023). Characterization and classification of Spanish honeydew and blossom honeys based on their antioxidant capacity. Antioxidants, 12.

2. (2023, September 10). 581. Available online: https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2002:010:0047:0052:EN:PDF.

3. FAO, and WHO (2019). Codex Alimentarius Commission—Procedural Manual, WHO. [27th ed.].

4. Differences between honeydew and blossom honeys: A review;Trends Food Sci. Technol.,2017

5. Usefulness of amino acid composition to discriminate between honeydew and floral honeys. Application to honeys from a small geographic area;Iglesias;J. Agric. Food Chem.,2004