Ultrastructural Analysis of a Forming Embryonic Embodiment in the Adult Zebrafish Optic Tectum Surviving in Organotypic Culture

Abstract

It has been shown that adult zebrafish are capable of regenerating regions of the central nervous system (CNS) after insult. Unlike in higher-order vertebrates where damage to the CNS leads to glial scar formation and permanent functional deficits, damage to the adult zebrafish CNS is transient and followed by nearly complete reconstitution of both function and anatomy. Our lab’s previous work has shown that explants of zebrafish optic tectum can survive in organotypic culture for up to 7 days, and that at 96 h in culture, regenerating cells of the tectum begin to form structures that resemble the embryonic neural tube seen in vertebrate development. The current project aims to elucidate the cellular and ultrastructural components of the formation of this neural tube-like structure using scanning and transmission electron microscopy. Our results show that after injury and cultivation for 96 h, the explants contained differentiating cells that were undergoing several cellular events, such as neovascularization, and rosette/cisternae formation, leading to the formation of a structure resembling the embryonic neural tube. Additionally, we demonstrate healthy cellular ultrastructures in both degenerated and regenerated areas of the explant.