Functional Characterization of the Nuclear Receptor Gene SaE75 in the Grain Aphid, Sitobion avenae

Author:

Zheng Haixia1,Yan Yi12,Wei Guohua12,Merchant Austin3,Gu Yaxin24,Zhou Xuguo3ORCID,Zhu Xun2ORCID,Zhang Yunhui2,Li Xiangrui2

Affiliation:

1. College of Plant Protection, Shanxi Agricultural University, Taigu, Jinzhong 030800, China

2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

3. Department of Entomology, University of Kentucky, Lexington, KY 40546, USA

4. College of Plant Science, Tibet Agriculture and Animal Husbandry University, Linzhi 860000, China

Abstract

Ecdysteroid hormones are key regulators of insect development and metamorphosis. Ecdysone-inducible E75, a major component of insect ecdysone signaling pathway, has been well characterized in holometabolous insects, however, barely in hemimetabolous species. In this study, a total of four full-length E75 cDNAs from the English grain aphid, Sitobion avenae, were identified, cloned, and characterized. The four SaE75 cDNAs contained 3048, 2625, 2505, and 2179 bp open reading frames (ORF), encoding 1015, 874, 856, and 835 amino acids, respectively. Temporal expression profiles showed that SaE75 expression was low in adult stages, while high in pseudo embryo and nymphal stages. SaE75 was differentially expressed between winged and wingless morphs. RNAi-mediated suppression of SaE75 led to substantial biological impacts, including mortality and molting defects. As for the pleiotropic effects on downstream ecdysone pathway genes, SaHr3 (hormone receptor like in 46) was significantly up-regulated, while Sabr-c (broad-complex core protein gene) and Saftz-f1 (transcription factor 1) were significantly down-regulated. These combined results not only shed light on the regulatory role of E75 in the ecdysone signaling pathway, but also provide a potential novel target for the long-term sustainable management of S. avenae, a devastating global grain pest.

Funder

China Agriculture Research System of MOF and MARA

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Insect Science

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