Fitness of Nutrition Regulation in a Caterpillar Pest Mythimna separata (Walker): Insights from the Geometric Framework

Abstract

In nature, plants can contain variable nutrients depending upon the species, tissue, and developmental stage. Insect herbivores may regulate their nutrient intake behaviorally and physio- logically when encountering different foods. This study examined the nutritional regulation of the oriental armyworm, Mythimna separata, for the first time. In one experiment, we allowed the cater-pillars to choose between two nutritionally balanced but complementary diets. The caterpillars did not randomly consume the paired foods, but instead chose between the nutritionally balanced but complementary diets. This intake behavior was found to change with their developmental stages. Furthermore, the nutrient concentrations in food significantly impacted the insect’s performance. In the other experiment, caterpillars were given one of eleven diets that reflected the different nutrient conditions in the field. The results showed that proteins were significantly associated with developmental time and fecundity. For example, by consuming protein-biased food, the caterpillars developed faster and produced more eggs. In contrast, carbohydrates were more strongly linked to lipid accumulation, and caterpillars accumulated more lipids when consuming the carbohydrate-biased food. Moreover, the caterpillars were also found to actively regulate their intake of proteins and carbohydrates based on food quality and to physiologically prepare for subsequent life stages. These findings enhance our understanding of how M. separata feeds and responds to different nutritional environments in the field, which could have implications for managing insect herbivores in agricultural settings.