Presence of Multiple Genetic Mutations Related to Insecticide Resistance in Chinese Field Samples of Two Phthorimaea Pest Species
Author:
Zhu Jiang12, Chen Ruipeng2, Liu Juan1, Lin Weichao1, Liang Jiaxin1, Nauen Ralf3ORCID, Li Suhua1, Gao Yulin2
Affiliation:
1. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 3. Crop Science Division, R&D, Pest Control, Bayer AG, Alfred-Nobel-Str. 50, 40789 Monheim, Germany
Abstract
Potatoes hold the distinction of being the largest non-cereal food crop globally. The application of insecticides has been the most common technology for pest control. The repeated use of synthetic insecticides of the same chemical class and frequent applications have resulted in the emergence of insecticide resistance. Two closely related pests that feed on potato crops are the potato tuber moth, Phthorimaea operculella, and the tomato leafminer, Phthorimaea absoluta (syn. Tuta absoluta). Previous studies indicated the existence of insecticide resistance to various classes of insecticides including organophosphates, carbamates, and pyrethroids in field populations of P. operculella and P. absoluta. However, the exact mechanisms of insecticide resistance in P. operculella and to a lesser extent P. absoluta remain still poorly understood. Detecting resistance genotypes is crucial for the prediction and management of insecticide resistance. In this study, we identified multiple genetic mutations related to insecticide resistance in two species of Phthorimaea. An unexpected genetic divergence on target-site mutations was observed between P. operculella and P. absoluta. Three mutations (A201S, L231V, and F290V) in Ace1 (acetylcholinesterase), four mutations (M918T, L925M, T928I, and L1014F) in VGSC (voltage-gated sodium channel), and one mutation (A301S) in RDL (GABA-gated chloride channel) have been detected with varying frequencies in Chinese P. absoluta field populations. In contrast, P. operculella field populations showed three mutations (F158Y, A201S, and L231V) in Ace1, one mutation (L1014F) in VGSC at a lower frequency, and no mutation in RDL. These findings suggest that pyrethroids, organophosphates, and carbamates are likely to be ineffective in controlling P. absoluta, but not P. operculella. These findings contributed to a deeper understanding of the presence of target-site mutations conferring resistance to commonly used (and cheap) classes of insecticides in two closely related potato pests. It is recommended to consider the resistance status of both pests for the implementation of resistance management strategies in potatoes.
Funder
National Natural Science Foundation of China Guangdong Major Project of Basic and Applied Basic Research Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences
Reference60 articles.
1. Rout, G.R., and Peter, K.V. (2018). Genetic Engineering of Horticultural Crops, Academic Press. 2. China’s potato industry and potential impacts on the global market;Wang;Am. J. Potato Res.,2004 3. Amiri, A.N., and Bakhsh, A. (2019). An effective pest management approach in potato to combat insect pests and herbicide. 3 Biotech., 9. 4. The potato tuberworm: A literature review of its biology, ecology, and control;Rondon;Am. J. Potato Res.,2010 5. Alyokhin, A., Rondon, S.I., and Gao, Y.L. (2022). Insect Pests of Potato: Global Perspectives on Biology and Management, Academic Press. Available online: https://www.sciencedirect.com/book/9780123868954/insect-pests-of-potato.
|
|