Host Associations of Culex (Melanoconion) atratus (Diptera: Culicidae) and Culex (Melanoconion) pilosus from Florida, USA

Abstract

Characterizing the host-use patterns of mosquitoes is an essential component of understanding the transmission dynamics of mosquito-vectored pathogens. The host associations of two species of the medically important Culex subgenus Melanoconion, Culex atratus, and Culex pilosus are unknown or unclear, respectively. Both species have wide neotropical distributions. In the United States of America (USA), Culex pilosus occurs throughout the southeastern coastal plain, while Culex atratus is restricted to the southern Florida Peninsula. Using PCR-based blood meal analysis, we investigated the host associations of Culex atratus and Culex pilosus that were collected from Everglades National Park, Florida, USA We identified the host species of 208 Culex atratus and 168 Culex pilosus. Both species were narrowly associated with reptilian host species, particularly native and non-native lizards of the genus Anolis. Sampled Culex atratus exclusively fed on reptilian hosts, with >99% of blood meals derived from Anolis lizards. Culex pilosus fed predominantly from reptiles, but avian and mammalian blood meals were also detected. Of these, 92% of blood meals were derived from Anolis species. For both species, Anolis sagrei, an invasive exotic lizard in Florida, was the most frequently detected host species. These data indicate that Culex atratus and Culex pilosus are specialists of reptilian hosts, particularly Anolis lizards.