Abstract
The management of forest pests has become a significant challenge, particularly for wood borers, because they spend most of the time in the trunks or cones. The coneworm, Dioryctria abietella, is a representative of cone borers as its larvae feed on the cones of Pinaceae plants. The molecular mechanisms underlying the interactions between this species and host plants or habitats can assist in developing strategies for pest control. In this study, we extended the expression profiles of 32 odorant binding proteins (OBPs) in the reproductive tissues of D. abietella, revealing the detectable transcription of 29 genes. Using two DabiOBPs highly expressed in antennae (DabiOBP5 and DabiOBP14) as targets, six compounds with high affinities (dissociation constants < 13 μM) were identified through a reverse chemical ecology strategy, including insecticides widely used for the control of lepidopteran pests. Of these compounds, a floral volatile β-ionone and a pear-produced ester ethyl-(2E,4Z)-decadienoate may serve as behaviorally active compounds in D. abietella. The strong binding of DabiOBPs to insecticides suggested their involvement in insecticide resistance, reflecting sophisticated detoxification mechanisms of this moth. In the molecular simulations, DabiOBP14 possessed stronger interactions with the six ligands compared to DabiOBP5, in which a few key residues within the binding pockets were involved in the formation of hydrogen bonds. This study provides some valuable reference active compounds for the development of lures or repellents in D. abietella and unravels the putative roles of two antenna-dominant DabiOBPs in the perception of plant-derived odorants and insecticides.
Funder
Yunnan Fundamental Research Project
National Natural Science Foundation of China
Yunnan Provincial Support Plan for the Cultivation of High-level Talents Young Top-notch Talents
Reference51 articles.
1. Phylogenetic framework for Dioryctria (Lepidoptera: Pyralidae: Phycitinae) based on combined analysis of mitochondrial DNA and morphology;Du;Can. Entomol.,2005
2. Advances in research and control of Dioryctria pests in China;Song;For. Pest Dis.,2020
3. Challenges of pheromone-based mating disruption of Cydia strobilella and Dioryctria abietella in spruce seed orchards;Svensson;J. Pest Sci.,2018
4. (3Z,6Z,9Z,12Z,15Z)-Pentacosapentaene and (9Z,11E)-tetradecadienyl acetate: Sex pheromone of the spruce coneworm Dioryctria abietella (Lepidoptera: Pyralidae);Svensson;J. Appl. Entomol.,2012
5. Synthetic pheromones disrupt male Dioryctria spp. moths in a loblolly pine seed orchard;DeBarr;Can. Entomol.,2000
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