Microbiome of the Aphid Genus Dysaphis Börner (Hemiptera: Aphidinae) and Its Relation to Ant Attendance

Abstract

Among mutualistic relationships of aphids with other organisms, there are two that seem to be of major importance: trophobiosis with ants and endosymbiosis of bacteria. While the former is well studied, the latter is the subject of an increasing amount of research constantly revealing new aspects of this symbiosis. Here, we studied the possible influence of ant attendance on the composition of aphid microbiota on primary and secondary hosts exploited by the aphid genus Dysaphis. The microbiome of 44 samples representing 12 aphid species was studied using an Illumina HiSeq 4000 with the V3-V4 region of 16S rRNA. The results showed a higher abundance of common facultative symbionts (Serratia, Regiella, Fukatsuia) in aphid species unattended by ants, but also on secondary hosts. However, in colonies attended by ants, the general species composition of bacterial symbionts was more rich in genera than in unattended colonies (Wolbachia, Gilliamella, Spiroplasma, Sphingomonas, Pelomonas). The results indicate a huge variability of facultative symbionts without clear correlation with ant attendance or aphid species. The possibility of multiple routes of bacterial infection mediated by ant-made environmental conditions is discussed.