Affiliation:
1. Biological Control of Pests Research Unit, United States Department of Agriculture-Agricultural Research Service, 59 Lee Road, Stoneville, MS 38776, USA
2. Delta Research and Extension Center, Mississippi State University, 82 Stoneville Road, Stoneville, MS 38776, USA
Abstract
Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasispecies virus with three main variants, which, in association with the ectoparasitic mite, Varroa destructor, causes wing deformity, shortened abdomens, neurological impairments, and colony mortality in honey bees. The red imported fire ant, Solenopsis invicta Buren, is one of the most-invasive and detrimental pests in the world. In this study, we report the co-occurrence of DWV-like symptoms in S. invicta and DWV for the first time and provide molecular evidence of viral replication in S. invicta. Some alates in 17 of 23 (74%) lab colonies and 9 of 14 (64%) field colonies displayed deformed wings (DWs), ranging from a single crumpled wing tip to twisted, shriveled wings. Numerous symptomatic alates also exhibited altered locomotion ranging from an altered gait to the inability to walk. Deformed wings may prevent S. invicta alates from reproducing since mating only occurs during a nuptial flight. The results from conventional RT-PCR and Sanger sequencing confirmed the presence of DWV-A, and viral replication of DWV was confirmed using a modified strand-specific RT-PCR. Our results suggest that S. invicta can potentially be an alternative and reservoir host for DWV. However, further research is needed to determine whether DWV is the infectious agent that causes the DW syndrome in S. invicta.
Reference34 articles.
1. Lowe, S., Browne, M., Boudjelas, S., and De Poorter, M. (2004). 100 of the World’s Worst Invasive Alien Species. A Selection from the Global Invasive Species Database, The Invasive Species Specialist Group (ISSG) a Specialist Group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN).
2. Global invasion history of the fire ant Solenopsis invicta;Ascunce;Science,2011
3. Vinson, S.B. (1986). Economic Impact and Control of Social Insects, Praeger.
4. Impact of the invasion of the imported fire ant;Vinson;Insect Sci.,2013
5. The detection of honey bee (Apis mellifera)-associated viruses in ants;Payne;Sci. Rep.,2020