First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects-Reference-Cited by-同舟云学术

First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects

Published:2024-07-26 Issue:8 Volume:15 Page:566
ISSN:2075-4450
Container-title:Insects
language:en
Short-container-title:Insects

Author:

Dimitrov Roumen¹,Gouliamova Dilnora²^ORCID,Guéorguiev Borislav³^ORCID,Smith Maudy⁴,Groenewald Marizeth⁴,Boekhout Teun⁵⁶^ORCID

Affiliation:

1. Institute of Mathematics and Informatics, Bulgarian Academy of Sciences, G. Bonchev 8, 1113 Sofia, Bulgaria

2. The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, G. Bonchev 26, 1113 Sofia, Bulgaria

3. National Museum of Natural History, Bulgarian Academy of Sciences, bul. “Tsar Osvoboditel” 1, 1000 Sofia, Bulgaria

4. Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

5. The Yeast Foundation, 1015 JR Amsterdam, The Netherlands

6. College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia

Abstract

In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.

Funder

Bulgarian National Science Fund

Publisher

MDPI AG

Link

https://www.mdpi.com/2075-4450/15/8/566/pdf

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