Self-Selection of Feeding Substrates by Tenebrio molitor Larvae of Different Ages to Determine Optimal Macronutrient Intake and the Influence on Larval Growth and Protein Content

Abstract

Nutrient self-selection was used to determine the optimal uptake of macronutrients by the yellow mealworm (Tenebrio molitor) larvae. The selection study consisted of four combinations of eight pelleted substrates from a total choice of 25, available to the larvae in a multiple-choice arena. In order to be able to determine the nutrient requirements as a function of the larvae age, six, eight and tenweekold larvae were used for the experiment. The larvae were free to choose between the different feeds for a period of two weeks. Rearing took place at 27 °C, 75% relative humidity and under dark conditions. The optimal ratios of macronutrients were 67.3 to 71.5% for carbohydrates, 19.9 to 22.8% for proteins and 8.6 to 10.0% for lipids to ensure the best results. Biomass growth, food intake and conversion were positively influenced to a significant extent by carbohydrate intake. The protein content, too, varied according to the macronutrient intake and substrate composition; a higher protein consumption increased the larval protein content. Wheat bran and flour, oat bran and flakes, maize hulls, lupine flour and potato flakes, in particular, were considered suitable substrates for the feeding and rearing of Tenebrio molitor larvae and highlighted that these larvae preferred a grain-based diet.