Microbiomes of Two Pest Fly Species of Pennsylvania Mushroom Houses

Author:

Sakamoto Joyce M.1ORCID,Shikano Ikkei2ORCID,Rasgon Jason L.13ORCID

Affiliation:

1. Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA

2. CTAHR Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA

3. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA

Abstract

Mushroom cultivation vastly improves the yield of mushrooms under optimized, controlled conditions, but may be susceptible to opportunistic colonization by pest species that can establish themselves, as well as the pathogens and pests they may transmit. Here, we describe our investigation into the bacterial communities of adult Lycoriella ingenua (Diptera: Sciaridae) and Megaselia halterata (Diptera: Phoridae) collected from button mushroom (Agaricus bisporus) production houses in Pennsylvania. We collected adult flies and sequenced the hypervariable v4 region of the bacterial 16S rRNA using the Illumina MiSeq. The most abundant bacterial genus detected in both species was Wolbachia, but phylogenetic analysis revealed that the infections are from different clades. Future studies include the characterization of Wolbachia infections on fly behavior and biology, comparison of microbial diversity of fly species colonizing wild mushrooms, and other microbiota that may contribute to the success of certain pest fly species.

Funder

United States Department of Agriculture (USDA) National Institute of Food and Agriculture and Hatch Appropriations

Giorgi Mushroom Research Fund

Dorothy Foehr Huck and J. Lloyd Huck endowment

Publisher

MDPI AG

Reference38 articles.

1. Competition in Natural Populations of Mycophagous Drosophila;Grimaldi;Ecology,1984

2. Fungal Hosts of Mycetophilids (Diptera: Sciaroidea Excluding Sciaridae): A Review;Jakovlev;Mycology,2012

3. Biodiversity and Ecology of Mycophagous Diptera in Northeastern Ohio;Bunyard;Proc. Entomol. Soc. Wash.,2003

4. Stamets, P., and Chilton, J.S. (1983). The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home, Western Distribution by Homestead Book Co.

5. Willette, A., Van Slambrook, L., and Hollingsworth, C. (2023). Mushroom Pests: Mushroom-Mushroom Fly. Pacific Northwest Insect Management Handbook [Online], Oregon State University.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3