An Oil-Based Adjuvant Improves Immune Responses Induced by Canine Adenovirus-Vectored Vaccine in Mice
Author:
Broutin Manon12ORCID, Costa Fleur1, Peltier Sandy2, Maye Jennifer2, Versillé Nicolas2, Klonjkowski Bernard1
Affiliation:
1. UMR Virologie, INRAE, ANSES, EnvA, 94700 Maisons-Alfort, France 2. SEPPIC Paris La Défense, 92250 La Garenne Colombes, France
Abstract
There is a significant need for highly effective vaccines against emerging and common veterinary infectious diseases. Canine adenovirus type 2 (CAV2) vectors allow rapid development of multiple vaccines and have demonstrated their potential in animal models. In this study, we compared the immunogenicity of a non-replicating CAV2 vector encoding the rabies virus glycoprotein with and without MontanideTM ISA 201 VG, an oil-based adjuvant. All vaccinated mice rapidly achieved rabies seroconversion, which was associated with complete vaccine protection. The adjuvant increased rabies antibody titers without any significant effect on the anti-CAV2 serological responses. An RT2 Profiler™ PCR array was conducted to identify host antiviral genes modulated in the blood samples 24 h after vaccination. Functional analysis of differentially expressed genes revealed the up-regulation of the RIG-I, TLRs, NLRs, and IFNs signaling pathways. These results demonstrate that a water-in-oil-in-water adjuvant can shape the immune responses to an antigen encoded by an adenovirus, thereby enhancing the protection conferred by live recombinant vaccines. The characterization of early vaccine responses provides a better understanding of the mechanisms underlying the efficacy of CAV2-vectored vaccines.
Subject
Virology,Infectious Diseases
Reference40 articles.
1. Factors Which Contribute to the Immunogenicity of Non-Replicating Adenoviral Vectored Vaccines;Coughlan;Front. Immunol.,2020 2. Replication-Incompetent Adenoviral Vaccine Vector Elicits Effective Anti- Immunode®ciency-Virus Immunity;Shiver;Nature,2002 3. Bouet-Cararo, C., Contreras, V., Caruso, A., Top, S., Szelechowski, M., Bergeron, C., Viarouge, C., Desprat, A., Relmy, A., and Guibert, J.-M. (2014). Expression of VP7, a Bluetongue Virus Group Specific Antigen by Viral Vectors: Analysis of the Induced Immune Responses and Evaluation of Protective Potential in Sheep. PLoS ONE, 9. 4. Immune Responses in Pigs Induced by Recombinant Canine Adenovirus 2 Expressing the Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus;Zhou;Vet. Res. Commun.,2010 5. Qin, J., Huang, H., Ruan, Y., Hou, X., Yang, S., Wang, C., Huang, G., Wang, T., Feng, N., and Gao, Y. (2012). A Novel Recombinant Peste Des Petits Ruminants-Canine Adenovirus Vaccine Elicits Long-Lasting Neutralizing Antibody Response against PPR in Goats. PLoS ONE, 7.
|
|