The Influence of Physical Treatments on Seed Germination and Seedling Development of Spruce (Picea abies [L.] Karst.)

Abstract

The aim of this research was to investigate the influence of an electric field and gamma radiation upon the germination of spruce seeds. In order to carry out the research, spruce seeds from different provenances have been subjected to different treatments: electric field (EF) with 10 V, 30 V, and 50 V voltages and intensity of E = 266V/m, exposure time of 15 and 35 min, and gamma (G) radiation with several treatments (1 Gy-31 min, 1.5 Gy-46 min, 2 Gy-62 min, and 6 Gy-186 min). Under the influence of EF, the best results upon seed germination (80.83%) were recorded when seeds were treated with 30 V for 15 min, for all provenances investigated. Regarding gamma radiation, the highest germination percentage (87.50%) was achieved in T5G when seeds were subjected to 6 Gy for 186 min. It was also considered the interaction between seeds origin and the different EF and G treatments applied to the seeds to induce germination and further seedlings’ development. The results obtained after seeds were exposed to gamma radiation came out on top compared to electric field treatments, both for the germination and seedlings’ height.