Foliar Application of Selenium Reduces Cadmium Accumulation in Walnut Seedlings

Abstract

Cadmium (Cd) and selenium (Se) could jointly affect plant growth. To investigate the affect of Se on the Cd accumulation in Juglans regia and the physiological mechanism by which Se regulates Cd-induced oxidative stress, in this study, the effects of different foliar application doses of Se (0 (Se0), 20 (Se20), and 200 (Se200) μM) on J. regia (variety Xinfeng) seedlings under Cd stress (5 mM) were determined. The results show that exogenous application of Se (Se20 and Se200) increased walnut biomass compared with Se0 under Cd stress. Under Cd stress, exogenous application of 20 μM Se increased the catalase (CAT), peroxidase (POD), and ascorbate oxidase (AAO) activities in walnut roots and the CAT and AAO activities in walnut leaves, and exogenous application of 200 μM Se increased the CAT, POD, and AAO activities in walnut roots. Furthermore, under Cd stress, exogenous application of 20 and 200 μM Se both decreased the contents of superoxide (O2•−), hydrogen peroxide (H2O2), and malondialdehyde (MDA) in walnut roots and the content of MDA in walnut leaves. Moreover, application of 20 and 200 μM Se both reduced the accumulation of Cd in the root, wood, bark, and leaves of walnuts, and application of 200 μM Se enhanced Se concentration in the root, wood, bark, and leaves. Overall, exogenous application of Se, especially 200 μM Se, could reduce Cd accumulation and enhance CAT, POD, and AAO activities in Cd-stressed walnut roots, thus alleviating Cd stress. This study provides technical guidance for reducing the effects of Cd stress on walnut growth.