Development and Experimental Evaluation of Some Silver Nanoparticles with Antimicrobial Potential
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Published:2023-04-14
Issue:4
Volume:11
Page:1212
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ISSN:2227-9717
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Container-title:Processes
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language:en
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Short-container-title:Processes
Author:
Velescu Bruno Ștefan1, Ilie Marina Ionela1, Amzăr Anca Ioana1, Lupașcu Raluca Elisabeta1ORCID, Marandiuc Ilinca Mihaela1, Apetroaei Miruna-Maria1, Arsene Andreea Letiția1ORCID, Blejan Emilian Ionuț1, Nedea Octvian Alexandru2, Fistos Toma3, Fierăscu Radu Claudiu3, Bărbuceanu Florica4ORCID, Țoca Cristina4, Fierăscu Irina3ORCID, Udeanu Denisa Ioana1ORCID, Ghica Manuela1, Drăgănescu Doina1, Cobelschi Pavel Călin5
Affiliation:
1. Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956 Bucharest, Romania 2. Faculty of Biotechnical Systems Engineering, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania 3. National Institute for Research and Development in Chemistry and Petrochemistry, 060021 Bucharest, Romania 4. National Institute for Diagnosis and Animal Health, 050557 Bucharest, Romania 5. Faculty of Medicine, Transilvania University, 500019 Brașov, Romania
Abstract
By adjusting the synthesis process, silver nanoparticles (AgNp) of various shapes, sizes, and structures can be obtained, all of which have a substantial impact on the biological effect, notably, the regulation of antibacterial activity in the present circumstances of growing bacterial resistance. Due to their relatively small size, nanoparticles may be disseminated evenly throughout the body of the experimental animal, even at low doses, and exert more potent antibacterial activities. Our research was centered on the synthesis, production, and biological evaluation of antibacterial silver nanoparticles. Using the Turkevich method, we were able to effectively synthesize and characterize nanoscale silver particles, with an average crystallite size of 9.49 nm. We examined their acute toxicity and pharmacokinetic characteristics in rats after administering a single dosage. In addition, we evaluated the biological effect of topical AgNp suspension on the progression of burn-type lesions in the experimental animals. The pharmacokinetic profile demonstrated that the plasma concentration of silver nanoparticles, as well as their clearance rate, and dispersion throughout the body, are significantly enhanced in large rodent species. The restorative effect of synthesized silver nanoparticles in the form of a suspension in distilled water was corroborated by the values of the hematological parameters. These results demonstrated an intense stimulation of the cellular and molecular processes of the local immune defense, which has resulted in significantly faster regeneration in the AgNp-treated group.
Funder
“Carol Davila” University of Medicine and Pharmacy Bucharest Ministry of Research and Innovation within PNCDI III
Subject
Process Chemistry and Technology,Chemical Engineering (miscellaneous),Bioengineering
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