Transmission Dynamics of Imported Vaccine-Origin PRRSV-2 within and between Commercial Swine Integrations in Hungary-Reference-Cited by-同舟云学术

Transmission Dynamics of Imported Vaccine-Origin PRRSV-2 within and between Commercial Swine Integrations in Hungary

Published:2023-10-02 Issue:19 Volume:13 Page:3080
ISSN:2076-2615
Container-title:Animals
language:en
Short-container-title:Animals

Author:

Jakab Szilvia¹²^ORCID,Bányai Krisztián¹²³,Bali Krisztina¹²^ORCID,Nemes Imre⁴,Bálint Ádám⁵,Szabó István⁴^ORCID

Affiliation:

1. HUN-REN Veterinary Medical Research Institute, Hungária krt. 21., H-1143 Budapest, Hungary

2. National Laboratory for Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Hungária krt. 21., H-1143 Budapest, Hungary

3. Department of Pharmacology and Toxicology, University of Veterinary Medicine, István utca. 2., H-1078 Budapest, Hungary

4. National PRRS Eradication Committee, Keleti Károly utca. 24., H-1024 Budapest, Hungary

5. Veterinary Diagnostic Directorate, National Food Chain Safety Office, H-1143 Budapest, Hungary

Abstract

This study reports on the molecular epidemiology of Ingelvac-PRRS-MLV-associated cases in Hungary for the period 2020–2021. Field epidemiology investigations led the experts to conclude that imported pigs, which were shipped through transit stations in Denmark, introduced the vaccine virus. The movement of fatteners and the neglect of disease control measures contributed to the spread of the virus to PRRS-free pig holdings in the vicinity. Deep sequencing was performed to genetically characterize the genes coding for the virion antigens (i.e., ORF2 through ORF7). The study isolates exhibited a range of 0.1 to 1.8% nucleotide sequence divergence from the Ingelvac PRRS MLV and identified numerous polymorphic sites (up to 57 sites) along the amplified 3.2 kilo base pair genomic region. Our findings confirm that some PRRSV-2 vaccine strains can accumulate very high number of point mutations within a short period in immunologically naive pig herds.

Funder

National Laboratory for Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety

National Research, Development and Innovation Fund

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Link

https://www.mdpi.com/2076-2615/13/19/3080/pdf

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