Changes in the Soil Microbial Community Structure and Driving Factors during Post-Fire Recovery of the Larix gmelinii Rupr. Forest in Northern China

Abstract

Fire is crucial for shaping northern forest ecosystems and can affect soil microbial community structure. However, there are few studies on the long-term effects of forest fire disturbance on soil microbial community diversity. In this study, we employed high-throughput sequencing of 16S rRNA and ITS1 to assess variations in the abundance of bacterial and fungal communities in dominant populations at 1, 6, and 11 years post-fire. Furthermore, a comprehensive analysis was conducted to examine the relationship between soil microenvironmental changes and soil microbial communities after fire disturbances, considering soil physicochemical properties, including bulk density, moisture content, pH, organic carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, available potassium, and available phosphorus. We found that fire significantly increased soil pH, NO3−-N, AP, and AK contents, in which the content of NO3−-N basically recovered to the pre-fire level at 11 years after fire. The soil SOC and TN contents decreased significantly 1 year after the fire. However, compared to the unfired site, the SOC content essentially recovered 11 years after the fire, while TN content was still significantly higher 11 years after fire. Furthermore, fire changed the diversity and richness of soil microbial communities to some extent. PCoA and NMDS analyses suggested that the bacterial community structures in soil samples from different burned areas with different recovery periods exhibited similarity. However, notable differences were observed in the fungal community structures between the 1-year and 6-year post-fire study sites when compared to the unburned control site. Bacterial communities predominantly comprised Proteobacteria, Actinobacteria, and Acidobacteria, while fungal communities were mainly dominated by Ascomycota and Basidiomycota. RDA confirmed the significant roles of SOC, TN, and NO3−-N in affecting the diversity of soil microbial communities. Therefore, our study not only enhances our understanding of the long-term effects of forest fire disturbances on soil properties and soil microbial community structure, but also provides insights for further utilizing and controlling carbon and nitrogen content to regulate soil microbial activity and accelerate the recovery process of burned areas.