Affiliation:
1. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, Italy
2. Dipartimento Veterinario e Sicurezza degli Alimenti di Origine Animale ATS, Brescia, Viale Duca degli Abruzzi 15, 25124 Brescia, Italy
Abstract
Over the last few decades, several pestiviruses have been discovered in ruminants, pigs, and, more recently, in non-ungulate hosts. Consequently, the nomenclature and taxonomy of pestiviruses have been updated. The Tunisian sheep-like pestivirus (TSV, Pestivirus N) is an additional ovine pestivirus genetically closely related to classical swine fever virus (CSFV). In this study, during a survey of pestivirus infections in ovine farms in the Lombardy region of Northern Italy, we identified and isolated a pestivirus strain from a sheep that was found to belong to Pestivirus N species based on its genomic nucleotide identity. The sheep itself and its lamb were found to be persistently infected. We performed molecular characterization and phylogenetic analysis of three viral genomic regions (a fragment of 5′-UTR, partial Npro, and the whole E2 region). In conclusion, these results confirmed circulating TSV in Northern Italy after notification in Sicily, Italy, and France. Correlation with Italian, Tunisian, and French strains showed that detection might have resulted from the trading of live animals between countries, which supports the need for health control measures.
Funder
Italian Ministry of Health
Reference27 articles.
1. Economic impact of BVDV infection in dairies;Houe;Biologicals,2003
2. Pestivirus control programs: How far have we come and where are we going?;Moennig;Anim. Health Res. Rev.,2015
3. Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae;Smith;J. Gen. Virol.,2017
4. Postel, A., Smith, D.B., and Becher, P. (2021). Proposed Update to the Taxonomy of Pestiviruses: Eight Additional Species within the Genus Pestivirus, Family Flaviviridae. Viruses, 13.
5. (2024, April 20). ICTV Report. Flaviviridae, Genus: Pestivirus. Available online: https://ictv.global/taxonomy/taxondetails?taxnode_id=202203153&taxon_name=Pestivirus%20bovis.