Morphology and Viability of Pollen from Three Hardy Water Lilies and Their Cross-Compatibility with Nymphaea hybrid

Abstract

Owing to the wide variation in their morphological characteristics across diverse geographies, the identification and classification of plants in the Nymphaea genus are challenging. Therefore, the present study investigated the pollen morphological characteristics of hardy water lilies (N. ‘Rose Arey’, N. ‘Perry’s Fire Opal’, and N. ‘Peter Slocum’), their relationship with species classification and system evolution, and their cross-compatibility with three strains of Nymphaeahybrid (NH-1, NH-2, and NH-3), a tropical waterlily. Pollen of the hardy water lilies was single-grained, oblate, and 18.31–20.47 × 32.51–37.64 μm. The pollen apertures were of the ring-groove type, and the pollen exine ornamentation was rod- and tumour-shaped. Pollen grains of different species differed in size, the obviousness of tumour ornamentation, and the size and density of rod-like ornamentation; their germination rates also differed significantly. Viabilities of N. ‘Rose Arey’ and N. ‘Peter Slocum’ pollen were the highest and lowest, respectively. According to the artificial pollination results, all hybrid combinations except three (NH-1 × N. ‘Peter Slocum’, NH-2 × N. ‘Peter Slocum’, and NH-3 × N. ‘Peter Slocum’) bore seeds; combinations with NH-2 as the female parent and N. ‘Rose Arey’ as the male parent had the highest seed-setting rates.