Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium
-
Published:2022-11-28
Issue:12
Volume:11
Page:2349
-
ISSN:2076-3921
-
Container-title:Antioxidants
-
language:en
-
Short-container-title:Antioxidants
Author:
Tiboldi AkosORCID,
Hunyadi-Gulyas EvaORCID,
Wohlrab Peter,
Schmid Johannes A.ORCID,
Markstaller Klaus,
Klein Klaus Ulrich,
Tretter VerenaORCID
Abstract
Patients presenting with insufficient tissue oxygenation and impaired lung function as in acute respiratory distress syndrome (ARDS) frequently require mechanical ventilation with supplemental oxygen. Despite the lung being used to experiencing the highest partial pressure of oxygen during healthy breathing, the organ is susceptible to oxygen-induced injury at supraphysiological concentrations. Hyperoxia-induced lung injury (HALI) has been regarded as a second hit to pre-existing lung injury and ventilator-induced lung injury (VILI) attributed to oxidative stress. The injured lung has a tendency to form atelectasis, a cyclic collapse and reopening of alveoli. The affected lung areas experience oxygen conditions that oscillate between hyperoxia and hypoxia rather than remaining in a constant hyperoxic state. Mechanisms of HALI have been investigated in many animal models previously. These studies provided insights into the effects of hyperoxia on the whole organism. However, cell type-specific responses have not been dissected in detail, but are necessary for a complete mechanistic understanding of ongoing pathological processes. In our study, we investigated the effects of constant and intermittent hyperoxia on the lung endothelium from a mouse by an in vitro proteomic approach. We demonstrate that these oxygen conditions have characteristic effects on the pulmonary endothelial proteome that underlie the physiological (patho)mechanisms.
Funder
the Austria Science Funds
the Department of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna
the EU’s Horizon 2020 research and innovation program
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference51 articles.
1. Acute respiratory distress in adults;Lancet,1967
2. Advancing precision medicine for acute respiratory distress syndrome;Lancet Respir. Med.,2022
3. Recent advances in understanding acute respiratory distress syndrome;F1000Res,2018
4. Hyperoxic acute lung injury;Respir. Care,2013
5. Ventilator-induced injury: From barotrauma to biotrauma;Proc. Assoc. Am. Physicians,1998