Author:
Neşelioğlu Salim,Fırat Oğuz Esra,Erel Özcan
Abstract
Background: Ceruloplasmin plays an important role in the regulation of iron metabolism. Ceruloplasmin is an acute-phase protein known to have many metabolic effects. Its activity increases during infection, inflammation, and compensation of oxidation. In the current study, our aim is to develop a new method for the measurement of ferroxidase activity without requiring any chromogen. Methods: Venous blood samples were collected into serum separator tubes. Ferric iron ions formed by the enzyme ferroxidase were measured, both manually and fully automatically, at the 415 nm wavelength without using chromogen. These results were compared to conventional ferroxidase measurement methods and to the immunoturbidimetric ceruloplasmin measurement method. Results: The detection limit of the new assay was 14.8 U/L. The upper limit of the linearity was 1380 U/L. Precision values were calculated for high, medium, and low levels of ferroxidase activity in serum pool. The coefficient of variation was <5% for each level. Conclusion: In the present method, chromogens are not used. With its considerably low cost and short reaction time, this method is able to provide fast results, can be performed easily, and makes accurate measurements.
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference39 articles.
1. Burtis, C.A., and Ashwood, E.R. Tietz Textbook of Clinical Chemistry, 1994.
2. Serum levels of copper, ceruloplasmin and angiotensin converting enzyme among silicotic and non-silicotic workers;Beshir;Maced. J. Med. Sci.,2015
3. Changes in serum ceruloplasmin levels based on immunomodulatory treatments and melatonin supplementation in multiple sclerosis patients;Adamczyk-Sowa;Med. Sci. Monit. Int. Med. J. Exp. Clin. Res.,2016
4. Functional link between ferroxidase activity of ceruloplasmin and protective effect of apo-lactoferrin: Studying rats kept on a silver chloride diet;Kostevich;Biometals,2016
5. Ceruloplasmin and other copper binding components of blood plasma and their functions: An update;Linder;Metallomics,2016
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