Affiliation:
1. College of Food Science, South China Agricultural University, Guangzhou 510640, China
2. Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510640, China
3. Guangzhou Alchemy Biotechnology Co., Ltd., 139 Hongming Road Guangzhou Economic Technology Zone, Guangzhou 510760, China
Abstract
Ergothioneine (EGT), an exceptional antioxidant found ubiquitously across diverse living organisms, plays a pivotal role in various vital physiological regulatory functions. Its principal natural sources are mushrooms and animal liver tissues. Ganoderma spp., a traditional Chinese food and medicinal mushroom, boasts high concentrations of EGT. To advance the development of novel Ganoderma spp. strains with enhanced EGT yields, we employed an efficient Ganoderma spp. protoplasmic fusion system. Through molecular and biological characterization, we successfully generated seven novel fusion strains. Notably, fusion strain RS7 demonstrated a remarkable increase in mycelial EGT production (12.70 ± 1.85 mg/L), surpassing the parental strains FQ16 and FQ23 by 34.23% and 39.10%, respectively. Furthermore, in the context of the fruiting body, fusion strain RS11 displayed a notable 53.58% enhancement in EGT production (11.24 ± 1.96 mg/L) compared to its parental strains. Genomic analysis of the RS7, the strain with the highest levels of mycelial EGT production, revealed mutations in the gene EVM0005141 associated with EGT metabolism. These mutations led to a reduction in non-productive shunts, subsequently redirecting more substrate towards the EGT synthesis pathway. This redirection significantly boosted EGT production in the RS7 strain. The insights gained from this study provide valuable guidance for the commercial-scale production of EGT and the selective breeding of Ganoderma spp. strains.
Funder
The Key Area Research & Development Program of Guangdong Province
National Natural Science Foundation of China
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference56 articles.
1. Sur une base nouvelle retiree du seigle ergote, l’ergothioneine;Tanret;Rend. Acad. Sci.,1909
2. Role of ergothioneine in microbial physiology and pathogenesis;Cumming;Antioxid. Redox Signal.,2018
3. Ergothioneine, an adaptive antioxidant for the protection of injured tissues? A hypothesis;Halliwell;Biochem. Bioph. Res. Commun.,2016
4. Recent advances in understanding the in vivo distribution and metabolism of ergothioneine and its roles in disease prevention;Pan;Food Sci.,2019
5. Skin cells and tissue are capable of using l-ergothioneine as an integral component of their antioxidant defense system;Markova;Free Radic. Biol. Med.,2009