In Vitro Tracking of Human Umbilical Vein Endothelial Cells Using Ultra-Sensitive Quantum Dot-Embedded Silica Nanoparticles

Author:

Kim Jaehi1,Lee Sunray2,Lee Yeon Kyung2,Seong Bomi1,Kim Hyung-Mo1,Kyeong San3,Kim Wooyeon1,Ham Kyeongmin1,Pham Xuan-Hung1ORCID,Hahm Eunil1,Mun Ji Yeon2,Safaa Mukhtar Anthony2,Lee Yoon-Sik3,Jun Bong-Hyun1ORCID,Park Hyun-Sook2

Affiliation:

1. Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea

2. Stem Cell Niche Division, CEFO Research Center, Seoul 03150, Republic of Korea

3. School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea

Abstract

The nanoscale spatiotemporal resolution of single-particle tracking (SPT) renders it a powerful method for exploring single-molecule dynamics in living cells or tissues, despite the disadvantages of using traditional organic fluorescence probes, such as the weak fluorescent signal against the strong cellular autofluorescence background coupled with a fast-photobleaching rate. Quantum dots (QDs), which enable tracking targets in multiple colors, have been proposed as an alternative to traditional organic fluorescence dyes; however, they are not ideally suitable for applying SPT due to their hydrophobicity, cytotoxicity, and blinking problems. This study reports an improved SPT method using silica-coated QD-embedded silica nanoparticles (QD2), which represent brighter fluorescence and are less toxic than single QDs. After treatment of QD2 in 10 μg/mL, the label was retained for 96 h with 83.76% of labeling efficiency, without impaired cell function such as angiogenesis. The improved stability of QD2 facilitates the visualization of in situ endothelial vessel formation without real-time staining. Cells retain QD2 fluorescence signal for 15 days at 4 °C without significant photobleaching, indicating that QD2 has overcome the limitations of SPT enabling long-term intracellular tracking. These results proved that QD2 could be used for SPT as a substitute for traditional organic fluorophores or single quantum dots, with its photostability, biocompatibility, and superior brightness.

Funder

Ministry of Science and ICT

Ministry of Health & Welfare

Korean Fund for Regenerative Medicine

Konkuk University Researcher Fund

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Reference30 articles.

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Infrared Weak Dot Target Tracking Algorithm Based on Meta-Learning;2023 International Conference on Ambient Intelligence, Knowledge Informatics and Industrial Electronics (AIKIIE);2023-11-02

2. Silica Encapsulation of Hydrophobic Optical NP-Embedded Silica Particles with Trimethoxy(2-Phenylethyl)silane;Nanomaterials;2023-07-24

3. Functional Optical Nano/Micromaterials;International Journal of Molecular Sciences;2023-04-18

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