Novel Insights into the Contribution of Cyclic Electron Flow to Cotton Bracts in Response to High Light

Abstract

Cyclic electron flow around photosystem I (CEF-PSI) is shown to be an important protective mechanism to photosynthesis in cotton leaves. However, it is still unclear how CEF-PSI is regulated in non-foliar green photosynthetic tissues such as bracts. In order to learn more about the regulatory function of photoprotection in bracts, we investigated the CEF-PSI attributes in Yunnan 1 cotton genotypes (Gossypium bar-badense L.) between leaves and bracts. Our findings demonstrated that cotton bracts possessed PROTON GRADIENT REGULATION5 (PGR5)-mediated and the choroplastic NAD(P)H dehydrogenase (NDH)-mediated CEF-PSI by the same mechanism as leaves, albeit at a lower rate than in leaves. The ATP synthase activity of bracts was also lower, while the proton gradient across thylakoid membrane (ΔpH), rate of synthesis of zeaxanthin, and heat dissipation were higher than those of the leaves. These results imply that cotton leaves under high light conditions primarily depend on CEF to activate ATP synthase and optimize ATP/NADPH. In contrast, bracts mainly protect photosynthesis by establishing a ΔpH through CEF to stimulate the heat dissipation process.