Integrative Transcriptomic and Metabolomic Analysis Reveals the Molecular Mechanism of Red Maple (Acer rubrum L.) Leaf Coloring
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Published:2023-03-23
Issue:4
Volume:13
Page:464
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ISSN:2218-1989
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Container-title:Metabolites
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language:en
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Short-container-title:Metabolites
Author:
Luo Yuanyuan12, Deng Min3, Zhang Xia145, Zhang Damao145, Cai Wenqi145, Long Yuelin16, Xiong Xingyao17, Li Yanlin1458910ORCID
Affiliation:
1. College of Horticulture, Hunan Agricultural University, Changsha 410128, China 2. College of Oriental Science & Technology, Hunan Agricultural University, Changsha 410128, China 3. College of Agronomy, Hunan Agricultural University, Changsha 410128, China 4. Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha 410128, China 5. Hunan Mid-Subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center, Changsha 410128, China 6. College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, China 7. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China 8. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China 9. Kunpeng Institute of Modern Agriculture, Foshan 528200, China 10. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
Abstract
This study employed a combination of ultraviolet spectrophotometry, LC-ESI-MS/MS system, and RNA-sequencing technology; the extracts and isolation of total RNA from the red and yellow leaf strains of red maple (Acer rubrum L.) at different developmental stages were subjected to an intercomparison of the dynamic content of chlorophyll and total anthocyanin, flavonoid metabolite fingerprinting, and gene expression. The metabonomic results indicated that one hundred and ninety-two flavonoids were identified, which could be classified into eight categories in the red maple leaves. Among them, 39% and 19% were flavones and flavonols, respectively. The metabolomic analysis identified 23, 32, 24, 24, 38, and 41 DAMs in the AR1018r vs. AR1031r comparison, the AR1018r vs. AR1119r comparison, the AR1031r vs. AR1119r comparison, the AR1018y vs. AR1031y comparison, the AR1018y vs. AR1119y comparison, and the AR1031y vs. AR1119y comparison, respectively. In total, 6003 and 8888 DEGs were identified in AR1018r vs. AR1031r comparison and in the AR1018y vs. AR1031y comparison, respectively. The GO and KEGG analyses showed that the DEGs were mainly involved in plant hormone signal transduction, flavonoid biosynthesis, and other metabolite metabolic processes. The comprehensive analysis revealed that caffeoyl-CoA 3-O-methyltransferase (Cluster-28704.45358 and Cluster-28704.50421) was up-regulated in the red strain but down-regulated in the yellow strain, while Peonidin 3-O-glucoside chloride and Pelargonidin 3-O-beta-D-glucoside were up-regulated in both the red and yellow strains. By successfully integrating the analyses on the behavior of pigment accumulation, dynamics of flavonoids, and differentially expressed genes with omics tools, the regulation mechanisms underlying leaf coloring in red maple at the transcriptomic and metabolomic levels were demonstrated, and the results provide valuable information for further research on gene function in red maple.
Funder
Research Foundation of Education Bureau of Hunan Province, China the science and technology innovation Program of Hunan Province Key project of Hunan Provincial Department of Education The Forestry Science and Technology Innovation Foundation of Hunan Province for Distinguished Young Scholarship Open Project of Horticulture Discipline of Hunan Agricultural University National Innovation and Entrepreneurship Training Program for College Students Innovation and Entrepreneurship Training Program of Hunan Province for College Students
Subject
Molecular Biology,Biochemistry,Endocrinology, Diabetes and Metabolism
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