Cleft Lip and Palate in Four Full-Sib Puppies from a Single Litter of Staffordshire Bull Terrier Dogs: An Anatomical and Genetic Study
Author:
Ruszkowski Jakub J.1ORCID, Nowacka-Woszuk Joanna2ORCID, Nowak Tomasz2, Rozynek Jedrzej2, Serwanska-Leja Katarzyna1ORCID, Gogulski Maciej34, Kolodziejski Pawel5ORCID, Switonski Marek2ORCID, Zdun Maciej1ORCID, Szczerbal Izabela2ORCID
Affiliation:
1. Department of Animal Anatomy, Poznan University of Life Sciences, Wojska Polskiego 71C, 60-625 Poznan, Poland 2. Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland 3. Department of Preclinical Sciences and Infectious Diseases, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland 4. University Centre for Veterinary Medicine, Poznan University of Life Sciences, Szydłowska 43, 60-637 Poznan, Poland 5. Department of Animal Physiology, Biochemistry and Biostructure, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland
Abstract
Cleft lip and palate (CLP) is a well-known congenital defect in dogs, characterized by abnormal communication between the oral and nasal cavities. Its incidence rate is high and affects all dog breeds. The etiology of CLP is thought to be multifactorial, caused by both genetic and environmental factors. In this study, four puppies out of seven from a single litter of Staffordshire Bull Terrier dogs with craniofacial abnormalities were anatomically and genetically examined. Classical anatomical preparation, dyed-latex-injection of the arterial vessels, and cone-beam computed tomography were used. The puppies showed variations in their observable abnormalities: three of them had a complete cleft of the palate on both sides, while one puppy had a cleft on the right side only. Cytogenetic analysis showed a normal diploid chromosome number (2n = 78,XX or 78,XY) in the studied animals. Known genomic variants of CLP were examined in the ADAMTS20, DLX6, and MYH3 genes, but no mutations were identified. Further studies are needed to identify the breed-specific genetic variants associated with canine CLP.
Funder
Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences
Subject
General Veterinary,Animal Science and Zoology
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