Combined Role of Fe Nanoparticles (Fe NPs) and Staphylococcus aureus L. in the Alleviation of Chromium Stress in Rice Plants

Abstract

Chromium (Cr) is a toxic heavy metal whose high concentration in soil badly affects plant growth, photosynthesis, and overall yield. Metal-derived nano-particles and metal-resistant bacteria can strengthen the plant defense system against different abiotic stresses; however, little is known about the use of nanoparticles in conjunction with bacteria. This study examined the combined effect of Fe nanoparticles (Fe NPs) and a chromium-resistant bacterium Staphylococcus aureus, on rice plants grown on chromium saturated medium. Chromium stress reduced rice growth, biomass, and chlorophyll contents by causing oxidative damage leading to overproduction of electrolyte leakage, hydrogen peroxide, and malondialdehyde. Fe NPs significantly improved plant growth, biomass, yield, and photosynthetic activity by enhancing the chlorophyll contents and alleviating oxidative damage. Application of Fe NPs also reduced the uptake and accumulation of Cr in the plants by increasing the bioavailability of micronutrients to the plant. The Fe NPs decreased oxidative damage and enhanced the enzymatic and non-enzymatic activity in the plant to withstand Cr stress compared to the plants without Fe NPs treatments. The inoculation of rice plants with the chromium-resistant bacteria S. aureus further enhanced the positive impact of Fe NPs by transforming the toxic form of chromium (Cr6+) into a less toxic form of chromium (Cr3+). The bacterial inoculation reduced Cr uptake by plants through adsorption of Cr ions, resulting in decreased chromium ion bioavailability. At chromium level 100 mg/kg, the foliar application of Fe NPs from 0 to 20 mg/L increased the total chlorophyll contents from 2.8 to 3.9. The application of S. aureus further enhanced the chlorophyll contents from 4.4 to 5.4, respectively. The current study suggested that combining Fe NPs and S. aureus could be a viable strategy for reducing Cr toxicity and accumulation in rice plants and most likely other plants.