Characterization of Uncoated and Coated Fungal Mycelium-Based Composites from Water Hyacinth

Abstract

Mycelium-based composites are a promising avenue for innovating sustainable materials from the hyphae of fungi. This study focuses on the use of fibers from four local fungal species, namely, Pleurotus ostreatus, Pleurotus sajor-caju (Fr. Singer), Auricularia auricula-judae, and Schizophyllum commune Fr., to produce mycelium-based composites from water hyacinth. An inoculum of each of the mushroom species was cultivated on PDA medium at 25 and 30 °C to determine the optimal temperature based on the growth rate. The obtained optimal condition was used to grow the fungi on water hyacinth (WH) mixed with rice bran in different proportions (100% WH, 70% WH, and 50% WH) with various numbers of fungal inocula (10, 20, and 30 plugs). The obtained composites were coated with a solution of either starch, chitosan, or epoxy resin. Schizophyllum commune Fr. exhibited the highest growth rate and fiber density, with a growth rate of 1.45 ± 1.92 mm/day at 30 °C. Ten inocula of Schizophyllum commune Fr. incubated at 30 °C for seven days on a mixture of 50% WH and 50% rice bran gave the optimal composite. Coating the obtained composite with chitosan improved its mechanical properties, but coating it with epoxy resin improved its water absorbency. Buried in soil, the composite coated with a chitosan solution decomposed within 30 days. The results indicate that Schizophyllum commune Fr. can be used as a binder to produce mycelial composites on a substrate of WH mixed with rice bran. The implications of these results will enable the further development and tuning of mushroom-based materials, especially for the production of sustainable bio-construction materials derived from local mushrooms and bio-waste.