In Situ Fabrication of Polydeoxyribonucleotide-Impregnated Hydroxyapatite onto a Magnesium Surface

Abstract

In this study, in situ polydeoxyribonucleotide-impregnated hydroxyapatite (PDRN/HA) was coated on a magnesium (Mg) substrate to form a biocompatible HA layer by chemical conversion for effective PDRN delivery. The HA layer showed needle-like morphology, and the PDRN impregnation did not affect the coating structure. The loading amount of PDRN via the proposed in situ method was 2.5 times higher than that by the conventional dipping method. An in vitro cell proliferation test demonstrated that the PDRN loading was more effective through this one-step method than through the dipping method. The results indicate that in situ PDRN/HA coating can enhance the potential of Mg-based implants.