Author:
Xu Kongliang,Wang Bin,Si Chenlu,Lin Chaoping,Zheng Renchao,Zheng Yuguo
Abstract
As a type of important and versatile biocatalyst, amidase immobilization on solid materials has received broad attention with its relatively easy procedure and available reusability. However, current porous supports have suffered from limited loadings, and it is highly desired to develop a new type of material with abundant space so as to ensure a high loading of amidase. Here, graphene oxide was adopted as the support for amidase immobilization, which showed the highest loading capacity for amidase (~3000 mg/g) to date. To the best of our knowledge, it is the first case of amidase immobilized on graphene oxide. Through surface modulation via reducing the contents of oxygen-containing functional groups, activity recovery of immobilized amidase increased from 67.8% to 85.3%. Moreover, surface-modulated graphene oxide can efficiently uptake amidase under a wide range of pH, and the maximum loading can reach ~3500 mg/g. The resultant biocomposites exhibit efficient biocatalytic performance for asymmetric synthesis of a chiral amino acid (i.e., L-4-fluorophenylglycine, an intermediate of aprepitant).
Funder
National Key Research and Development Program of China
Natural Science Foundation of Zhejiang Province
Subject
Molecular Biology,Biochemistry
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献