New Insights into the Effects of Microbial Muramidase Addition in the Diets of Broiler Chickens

Author:

Amer Shimaa A.1ORCID,Farahat Mahmoud1,Gouda Ahmed2,Abdel-Wareth Ahmed A. A.3ORCID,Abdel-Warith Abdel-Wahab A.4,Younis Elsayed M.4ORCID,Elshopakey Gehad Elsaid5,Baher Weam Mohamed6,Saleh Gehan K.7,Davies Simon J.8ORCID,Attia Ghadeer A.1ORCID

Affiliation:

1. Department of Nutrition & Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt

2. Animal Production Department, Agricultural & Biological Research Division, National Research Center, Dokki, Cairo 11865, Egypt

3. Department of Animal and Poultry Production, Faculty of Agriculture, South Valley University, Qena 83523, Egypt

4. Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

5. Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 53511, Egypt

6. Food Hygiene Department, Animal Health Research Institute (AHRI) (Mansoura Branch) Agriculture Research Center (ARC), P.O. Box 246, Dokki, Giza 12618, Egypt

7. Biochemistry Department, Animal Health Research Institute (AHRI) (Mansoura Branch) Agriculture Research Center (ARC), P.O. Box 246, Dokki, Giza 12618, Egypt

8. School of Science and Engineering, National University of Ireland Galway Republic of Ireland, H91 TK33 Galway, Ireland

Abstract

The study aimed to explore how broiler chickens’ blood biochemistry, breast muscles’ fatty acid profile, growth, intestinal morphology, and immune status would be influenced by adding microbial muramidase (MUR) to the diet. Four hundred 3-day-old male broiler chickens were allocated to a completely randomized design consisting of four nutritional treatments (n = 100 per treatment, 10 chicks/replicate), each containing MUR at levels of 0 (control group), 200, 400, and 600 mg Kg−1 diet, with enzyme activity 0, 12,000, 24,000, and 36,000 LSU(F)/kg diet, respectively. The 35-day experiment was completed. The findings showed that adding MUR to broiler meals in amounts of 200, 400, or 600 mg/kg had no impact on growth performance (p > 0.05) during the periods of 4–10, 11–23, and 24–35 days of age. MUR supplementation quadratically impacted the feed conversion ratio of broiler chicks at 11 and 23 days of age (p = 0.02). MUR addition to the diet significantly and level-dependently enhanced the percentage of n−3 and n−6 polyunsaturated fatty acids (PUFA) in breast muscles (p ≤ 0.01), with no alterations to the sensory characteristics of the breast muscles. Dietary MUR increased most of the morphometric dimensions of the small intestine, with the best results recorded at the 200 and 400 mg Kg−1 levels. MUR supplementation at 200, 400, and 600 mg kg−1 linearly lowered the total cholesterol, triglycerides, and low-density lipoprotein cholesterol level (p < 0.01). Still, it significantly increased the high-density lipoprotein cholesterol and very-low-density lipoprotein cholesterol contents compared with the unsupplemented group. Compared to controls, there was a substantial rise in the blood concentration of total protein, albumin, globulin, IL10, complement 3, and lysozyme activity as MUR levels increased (p < 0.01). Moreover, MUR addition significantly increased the immunoexpression of lymphocyte subpopulation biomarkers. We could conclude that MUR can be added to broiler chicken diets up to 600 mg kg −1 to improve broiler chickens’ fatty acid profile in breast muscles, immunity, and blood biochemistry. MUR addition had no positive influence on the bird’s growth.

Funder

Zagazig University

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3