Electrospray Ionization—Mass Spectrometry Characterization of Pine Bark Extracts

Author:

Conde Mireia1ORCID,Solé Maria Mercè1ORCID,Sorolla Sílvia1,Casas Concepció1,Bacardit Anna1ORCID

Affiliation:

1. A3 Leather Innovation Center, Escola Politècnica Superior, Departament d’Informàtica i Enginyeria Industrial, Universitat de Lleida (UdL), 25003 Lleida, Spain

Abstract

This study explored the potential application of polyphenols from Pinus halepensis bark in leather tanning. The primary objective was to characterize these polyphenols. The extraction and atomization processes proved efficient, reducing moisture content to 7.4%, increasing tannin content from 26.2% to 45.1%, and reducing insoluble substances by 77.5%. High-performance liquid chromatography (HPLC) coupled with mass spectrometry identified and quantified various polyphenolic compounds, including (+)-catechin, (+)-taxifolin, protocatechuic acid, and procyanidin B2. Notably, tannic catechin dimers were detected. Lignin was effectively removed through filtration. Concentrations of protocatechuic acid, (+)-catechin, (+)-taxifolin, (−)-epicatechin, and procyanidin B1 were significantly higher in the extract than in the powder, with the extract showing 1214.3 mg/kg of protocatechuic acid, 2098.0 mg/kg of (+)-catechin, 4017.0 mg/kg of (+)-taxifolin, 2163.0 mg/kg of (−)-epicatechin, and 917.0 mg/kg of procyanidin B1.

Funder

Ministerio de Ciencia e Innovación

Publisher

MDPI AG

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