Microbial Diversity Impacts Non-Protein Amino Acid Production in Cyanobacterial Bloom Cultures Collected from Lake Winnipeg
Author:
Bishop Stephanie L.12ORCID, Solonenka Julia T.1, Giebelhaus Ryland T.134ORCID, Bakker David T. R.1, Li Isaac T. S.1ORCID, Murch Susan J.1ORCID
Affiliation:
1. Department of Chemistry, University of British Columbia, Syilx Okanagan Nation Territory, Kelowna, BC V1V 1V7, Canada 2. Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada 3. Department of Chemistry, University of Alberta, Edmonton, AB T6G 2N4, Canada 4. The Metabolomics Innovation Centre, Edmonton, AB T6G 2N4, Canada
Abstract
Lake Winnipeg in Manitoba, Canada is heavily impacted by harmful algal blooms that contain non-protein amino acids (NPAAs) produced by cyanobacteria: N-(2-aminoethyl)glycine (AEG), β-aminomethyl-L-alanine (BAMA), β-N-methylamino-L-alanine (BMAA), and 2,4-diaminobutyric acid (DAB). Our objective was to investigate the impact of microbial diversity on NPAA production by cyanobacteria using semi-purified crude cyanobacterial cultures established from field samples collected by the Lake Winnipeg Research Consortium between 2016 and 2021. NPAAs were detected and quantified by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) using validated analytical methods, while Shannon and Simpson alpha diversity scores were determined from 16S rRNA metagenomic sequences. Alpha diversity in isolate cultures was significantly decreased compared to crude cyanobacterial cultures (p < 0.001), indicating successful semi-purification. BMAA and AEG concentrations were higher in crude compared to isolate cultures (p < 0.0001), and AEG concentrations were correlated to the alpha diversity in cultures (r = 0.554; p < 0.0001). BAMA concentrations were increased in isolate cultures (p < 0.05), while DAB concentrations were similar in crude and isolate cultures. These results demonstrate that microbial community complexity impacts NPAA production by cyanobacteria and related organisms.
Funder
Natural Sciences and Engineering Research Council Canadian Foundation for Innovation
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