Diversity and Molecular Evolution of Antimicrobial Peptides in Caecilian Amphibians

Author:

Benítez-Prián Mario1ORCID,Lorente-Martínez Héctor1ORCID,Agorreta Ainhoa1ORCID,Gower David J.2ORCID,Wilkinson Mark3ORCID,Roelants Kim4,San Mauro Diego1ORCID

Affiliation:

1. Department of Biodiversity Ecology and Evolution, Faculty of Biological Sciences, Complutense University of Madrid, 28040 Madrid, Spain

2. Natural History Museum, London SW7 5BD, UK

3. Herpetology Lab, Natural History Museum, London SW7 5BD, UK

4. bDIV, Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Elsene, Belgium

Abstract

Antimicrobial peptides (AMPs) are key molecules in the innate immune defence of vertebrates with rapid action, broad antimicrobial spectrum, and ability to evade pathogen resistance mechanisms. To date, amphibians are the major group of vertebrates from which most AMPs have been characterised, but most studies have focused on the bioactive skin secretions of anurans (frogs and toads). In this study, we have analysed the complete genomes and/or transcriptomes of eight species of caecilian amphibians (order Gymnophiona) and characterised the diversity, molecular evolution, and antimicrobial potential of the AMP repertoire of this order of amphibians. We have identified 477 candidate AMPs within the studied caecilian genome and transcriptome datasets. These candidates are grouped into 29 AMP families, with four corresponding to peptides primarily exhibiting antimicrobial activity and 25 potentially serving as AMPs in a secondary function, either in their entirety or after cleavage. In silico prediction methods were used to identify 62 of those AMPs as peptides with promising antimicrobial activity potential. Signatures of directional selection were detected for five candidate AMPs, which may indicate adaptation to the different selective pressures imposed by evolutionary arms races with specific pathogens. These findings provide encouraging support for the expectation that caecilians, being one of the least-studied groups of vertebrates, and with ~300 million years of separate evolution, are an underexplored resource of great pharmaceutical potential that could help to contest antibiotic resistance and contribute to biomedical advance.

Funder

Complutense University of Madrid

Real Colegio Complutense at Harvard University

Publisher

MDPI AG

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